Biogenic zinc oxide nanoparticles from Saraca asoca: cytotoxicity, antioxidant, antimicrobial evaluation, and topical gel development
DOI:
https://doi.org/10.69857/joapr.v13i5.1300Keywords:
Zinc Oxide Nanoparticles, Saraca asoca, Green synthesis, Brine Shrimp cytotoxicity assay, antioxidant assay, anti-microbial assayAbstract
Background: Green synthesis of nanoparticles offers an eco-friendly and cost-effective alternative to conventional methods. Saraca asoca, a traditionally valued medicinal plant, contains bioactive compounds suitable for the fabrication of nanoparticles. This study reports the synthesis of zinc oxide nanoparticles (ZnO NPs) using Saraca asoca bark apozem and their incorporation into a biocompatible chitosan gel for topical applications. Methodology: ZnO NPs were synthesized via a green route using varying concentrations of zinc acetate (0.05–0.15 M) and Saraca asoca bark apozem (75–150 mg/mL), optimized through a 3² factorial design. The nanoparticles exhibited favorable formulation efficiency with % yield (40–46%), entrapment efficiency (55–62%), and drug loading (35–40%). Characterization confirmed nanoscale size (72.7–134.8 nm), negative zeta potential (–38.2 to –50.4 mV), UV–Vis absorbance (λmax = 366 nm), FTIR peaks of stabilizing –OH and –COO⁻ groups, and crystalline PXRD patterns. The optimized nanoparticles were incorporated into a 2% chitosan gel (Ash–ZnO NPs Cs gel). Results and Discussion: Antioxidant studies revealed strong free radical scavenging potential (IC₅₀ = 19.8 µg/mL). The Ash–ZnO NPs exhibited antimicrobial activity against Staphylococcus aureus and Candida albicans (MICs: 156.25 and 78.12 µg/mL). Cytotoxicity studies confirmed negligible toxicity (LC₅₀ > 1000 µg/mL). In vitro release studies demonstrated sustained and diffusion-controlled drug release, with Ash–ZnO NPs Cs gel achieving 96% release at 48 h compared to the burst release of silver nitrate gel. Conclusion: Saraca asoca-mediated ZnO NPs incorporated in chitosan gel represent a safe, stable, and multifunctional topical formulation. Their strong antioxidant, antimicrobial, biocompatible, and sustained release properties underscore their potential for wound healing and skin infection management.
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References
Bowen Y, Shi J. Recent advances in antioxidant nanomedicines. Innov Med Oncol, 2527, 2024 https://doi.org/10.36922/imo.2527
Eze FN, Okeke IN, Okezie UM, Eze PN. Green synthesis of zinc oxide nanoparticles using Piper guineense seed extract and evaluation of their antibacterial activity. Environ Nanotechnol Monit Manag, 22, 2024, 100804 https://doi.org/10.1016/j.enmm.2024.100804
Alharbi SA, Alobaid A, Alharbi NS, Kadaikunnan S. Biosynthesis of zinc oxide nanoparticles using Wodyetia bifurcata fruit peel extract: Characterisation, wound healing, and anticancer potential. Front Pharmacol, 15, 2024, 1435222 https://doi.org/10.3389/fphar.2024.1435222
Sudha A, Ramasamy P, Chandran S. Phytochemical-assisted synthesis of zinc oxide nanoparticles from Polyalthia longifolia leaf extract with potent antifungal efficacy. RSC Adv, 14(10), 2024, 5520–5530 https://doi.org/10.1039/D4RA01035C
Jain A, Bhise K. A review on inorganic UV filter zinc oxide against keratinocyte cancer and photoaging. Int J Adv Chem Res, 4(2), 2022, 332–336 https://doi.org/10.33545/26646781.2022.v4.i2e.119
Anu S, Scaria R, Jayaraj PS, Sudheesh. Comparative phytochemical profiling of different parts of Saraca asoca. Mater Today Proc, 2023 https://doi.org/10.1016/j.matpr.2023.11.083
Shamsa K, Bibi S, Haleem K, Waqar S, Mir A, Maalik S, Safia S, Hassan NS, Awwad HA, Ibrahium. Functional potential of chitosan–metal nanostructures: Recent developments and applications. Int J Biol Macromol, 136715, 2024 https://doi.org/10.1016/j.ijbiomac.2024.136715
Hameed H, Waheed A, Sharif MS, Saleem M, Afreen A, Tariq M, Kamal A, Al-Onazi W, Al Farraj DA, Ahmad S, Mahmoud RM. Green synthesis of zinc oxide nanoparticles from green algae and their assessment in various biological applications. Micromachines, 14(5), 2023, 928 https://doi.org/10.3390/mi14050928
Jain A, Bhise K. Green synthesis methods for metallic zinc oxide nanoparticles. In: Advances in Chemical and Materials Engineering Book Series, 2024, pp. 63–90 https://doi.org/10.4018/979-8-3693-6240-2.ch003
Rajeshkumar S, Bharath LV. Mechanism of plant-mediated synthesis of silver nanoparticles — a review on biomolecules involved, characterisation, and antibacterial activity. Chem Biol Interact, 273, 2017, 219–227 https://doi.org/10.1016/j.cbi.2017.06.019
Prakash MVD, Sampath S, Amudha K, Nadeem A, Lopes BS, Durga B, Muthupandian S. Eco-friendly green synthesis of copper nanoparticles from Tinospora cordifolia leaves: Optical properties with biological evaluation. Mater Technol, 38(1), 2023 https://doi.org/10.1080/10667857.2023.2247908
Vishnu K, Kumar S, Francis SP, Princy VM, Varghese R. Green synthesis, structural insights, and antimicrobial potential of zinc oxide nanoparticles synthesised via sustainable method. Curr Phys Chem, 2024 https://doi.org/10.2174/0118779468329256241007070941
Gupta R, Xie HJ, Sarkar M, Chen Y. Design of experiment (DOE) for optimisation of PLGA nanoparticles. FASEB J, 36(S1), 2022 https://doi.org/10.1096/fasebj.2022.36.s1.r6197
Afrasiabi S, Bahador A, Partoazar A. Combinatorial therapy of chitosan hydrogel-based zinc oxide nanocomposite attenuates the virulence of Streptococcus mutans. BMC Microbiol, 21(1), 2021 https://doi.org/10.1186/s12866-021-02128-y
Abdelmonem R, Elhabal SF, Abdelmalak NS, El-Nabarawi MA, Teaima MH. Formulation and characterisation of acetazolamide/carvedilol niosomal gel for glaucoma treatment: In vitro and in vivo study. Pharmaceutics, 13(2), 2021, 1–20 https://doi.org/10.3390/pharmaceutics13020221
Sugitha SKJ, Latha RG, Venkatesan R, Kim SC, Vetcher A, Khan MR. Green synthesis of Al–ZnO nanoparticles using Cucumis maderaspatanus plant extracts: Analysis of structural, antioxidant, and antibacterial activities. Nanomaterials, 2024 https://doi.org/10.3390/nano14020312
Thirumoorthy G, Balasubramaniam O, Kumaresan P, Muthusamy P, Subramani K. Tetraselmis indica-mediated green synthesis of zinc oxide nanoparticles and evaluating its antibacterial, antioxidant, and hemolytic activity. Bionanosci, 11(6), 2021, 1110–1122 https://doi.org/10.1007/s12668-021-00892-3
Fouda A, Saied E, Eid AM, Kouadri F, Alemam A, Hamza M, Alharbi MA, Elkelish AA, Hassan S. Green synthesis of zinc oxide nanoparticles using an aqueous extract of Punica granatum for antimicrobial and catalytic activity. J Funct Biomater, 14(4), 2023, 205 https://doi.org/10.3390/jfb14040205
Hashem A, El-Sayyad GS. Antimicrobial and anticancer activities of biosynthesized bimetallic silver–zinc oxide nanoparticles using pomegranate peel extract. Biomass Convers Biorefin, 2023 https://doi.org/10.1007/s13399-023-04781-z
Chen Y, Zhang L, Wu Y, Huang W, Luo Z, Li Y, Qi Y, Liu X, Shen G, Liu S, Tao S. Cytotoxicity and epithelial barrier toxicity of fine particles from residential biomass pellet burning. Environ Sci Technol, 58(40), 2024, 17786–17796 https://doi.org/10.1021/acs.est.4c04687
Sukkum C, Lekklar C, Chongsri K, Deeying S, Srisomsap C, Surapanich N, Kanjanasingh P, Hongthong S. Anti-cancer activity and brine shrimp lethality assay of the extracts and isolated compounds from Garcinia schomburgkiana Pierre. J Appl Pharm Sci, 15(3), 2025, 241–247 https://doi.org/10.7324/JAPS.2025.209800
Muthukrishnan S, Ramachandran A. Ethnobotanical study of the medicinal plants used by rural communities in the foothill villages of the Alagar Hills region, Eastern Ghats, Tamil Nadu, India. Ethnobot Res Appl, 30, 2025, 1–41 https://doi.org/10.32859/era.30.6242
Hassan HA, Sedky NK, Nafie MS, Mahdy NK, Fawzy IM, Fayed TW, Preis E, Bakowsky U, Fahmy S. Sustainable nanomedicine: Enhancement of Asplatin’s cytotoxicity using green-synthesised zinc oxide nanoparticles formed via microwave-assisted and gambogic acid-mediated processes. Molecules, 29(3), 2024 https://doi.org/10.3390/molecules29030712
Ashraf H, Meer B, Iqbal J, Ali J, Andleeb A, Butt H, Zia M, Mehmood A, Nadeem M, Drouet S, Blondeau J, Giglioli-Guivarc’h N, Liu C, Hano C, Abbasi B. Comparative evaluation of chemically and green synthesised zinc oxide nanoparticles: Their in vitro antioxidant, antimicrobial, cytotoxic, and anticancer potential. J Nanostruct Chem, 12(4), 2022, 789–802 https://doi.org/10.1007/s40097-022-00488-8
Islam MJ, Khatun N, Bhuiyan RH, Sultana S, Shaikh MAA, Bitu MNA, Chowdhury F, Islam S. Psidium guajava leaf extract-mediated green synthesis of silver nanoparticles and its application in antibacterial coatings. RSC Adv, 13(28), 2023, 19164–19172 https://doi.org/10.1039/d3ra03381c
Sandeep NC, Abishad P, Vinod VK, Karthikeyan A, Juliet S, Kurkure N, Barbuddhe S, Rawool D, Vergis J. Evaluation of ZnO nanoparticles from ‘Monsooned Malabar Robusta Coffee’ husk as a potential antioxidant and biocidal candidate. J Drug Deliv Sci Technol, 88, 2024, 105665 https://doi.org/10.1016/j.jddst.2024.105665
Hameed H, Waheed A, Sharif MS, Saleem M, Afreen A, Tariq M, Kamal A, Al-Onazi W, Al Farraj DA, Ahmad S, Mahmoud RM. Green synthesis of zinc oxide nanoparticles from green algae and their assessment in various biological applications. Micromachines, 14(5), 2023, 928 https://doi.org/10.3390/mi14050928
Yashni G, Willy K, Al-Gheethi A, Mohamed R, Mohd Salleh SNA, Amir Hashim MA. A review on green synthesis of ZnO nanoparticles using Coriandrum sativum leaf extract for degrading dyes in textile wastewater. IOP Conf Ser Mater Sci Eng, 991, 2020, 012112 https://doi.org/10.1088/1757-899X/991/1/012112
Baran M, Keskin C, Baran A, Hatipoğlu A, Yıldıztekin M, Küçükaydın S, Kurt K, Hosgören H, Sarker M, Sufianov A, Beylerli O, Khalilov R, Eftekhari A. Green synthesis of silver nanoparticles from Allium cepa L. peel extract and their antioxidant, antipathogenic, and anticholinesterase activity. Molecules, 28(5), 2023, 2310 https://doi.org/10.3390/molecules28052310
Bashir I, Gillani SS, Majeed F. Plant-extract of Mimusops elengi leaves and flower-mediated ZnO nanoparticles: Synthesis, characterisation, and biomedical applications. Sci Inq Rev, 7(2), 2023, 55–68 https://doi.org/10.32350/sir.72.04
Jain A, Bhise K. Nano-pharmacokinetics and pharmacodynamics of green-synthesised ZnO nanoparticles: A pathway to safer therapeutic applications. Xenobiotica, 55(3), 2025, 1–23 https://doi.org/10.1080/00498254.2025.2505062
Khatami M, Sharifi I, Nobre MAL, Zafarnia N, Aflatoonian MR. Waste-grass-mediated green synthesis of silver nanoparticles and evaluation of their anticancer, antifungal and antibacterial activity. Green Chem Lett Rev, 11(2), 2018, 125–134 https://doi.org/10.1080/17518253.2018.1444797
Balogun F, Ashafa A. Green-synthesised zinc oxide nanoparticles from aqueous root extract of Dicoma anomala mitigate free radicals and diabetes-linked enzymes. Nanoscience Nanotechnol Asia, 2020 https://doi.org/10.2174/2210681210666200117150727
Ayon SA, Billah M, Hossain MN. Effect of annealing temperature on structural, optical, and photocatalytic properties of modified sol–gel–driven ZnO nanoparticles. Surf Interface Anal, 2023 https://doi.org/10.1002/sia.7203
Fouda A, Saied E, Eid AM, Kouadri F, Alemam AM, Hamza MF, et al. Green synthesis of zinc oxide nanoparticles using an aqueous extract of Punica granatum for antimicrobial and catalytic activity. J Funct Biomater, 14(4), 2023, 205 https://doi.org/10.3390/jfb14040205
Dwivedi SD, Singh D, Singh MR. A Piper nigrum Based zinc oxide nanoparticles for anti-arthritic and antioxidant activity. J. Appl. Pharm. Res., 12, 51–9 (2024) https://doi.org/10.69857/joapr.v12i5.727.
Thenmozhi JR, Shahanaz S, Manuel SGA. Green synthesis of Zno nanoparticles using peels of Citrus limetta and evaluation of their antibacterial activity. J. Appl. Pharm. Res., 11, 27–34 (2023) https://doi.org/10.18231/j.joapr.2023.11.4.27.34.
Abdelghany T, Al-Rajhi AM, Yahya R, Bakri MM, Al Abboud MA, Qanash H, et al. Phytofabrication of zinc oxide nanoparticles with advanced characterisation and their antioxidant, anticancer, and antimicrobial activity. Biomass Convers Biorefin, 2022 https://doi.org/10.1007/s13399-022-02817-7
Moalwi A, Kamat K, Muddapur UM, Aldoah B, Alwadai H, Alamri A, et al. Green synthesis of zinc oxide nanoparticles from Wodyetia bifurcata fruit peel extract: Multifaceted potential in wound healing, antimicrobial, antioxidant, and anticancer applications. Front Pharmacol, 15, 2024, 1435222 https://doi.org/10.3389/fphar.2024.1435222
Sarnatskaya V, Shlapa Y, Kolesnik D, Lykhova O, Klymchuk D, Solopan S, et al. Bioactivity of cerium dioxide nanoparticles as a function of size and surface features. Biomater Sci, 2024 https://doi.org/10.1039/d3bm01900d
Tenchov R, Sasso JM, Zhou Q. Pegylated lipid nanoparticle formulations: Immunological safety and efficiency perspective. Bioconjug Chem, 2023 https://doi.org/10.1021/acs.bioconjchem.3c00174
Jeyachandran S, Giri J, Alarifi A. Biogenic synthesis of Bacillus lentus-mediated silver nanoparticles and its multifaceted applications in antibacterial, anti-biofilm, anti-larvicidal, and anticancer activities. Green Chem Lett Rev, 18(1), 2025 https://doi.org/10.1080/17518253.2025.2503730
Chinnaraj S, Palani V, Maluventhen V, Chandrababu R, Soundarapandian K, Kaliannan D, Arumugam M. Silver nanoparticle production mediated by Goniothalamus wightii extract: Characterization and their potential biological applications. Part Sci Technol, 41(4), 2022, 517–531 https://doi.org/10.1080/02726351.2022.2123752
Rahmayeni R, Oktavia Y, Stiadi Y, Arief S, Zulhadjri Z. Spinel ferrite of MnFe₂O₄ synthesised in Piper betle Linn extract media and its application as photocatalysts and antibacterial. J Dispersion Sci Technol, 42(3), 2020, 465–474 https://doi.org/10.1080/01932691.2020.172101
Karthik R, Suresh S, Arumugam P, Sudhakar U, Prabakaran M, Alharbi SA. Microwave-assisted green synthesis of zinc oxide nanoparticles using Azadirachta indica leaf extract and their antimicrobial and wound healing activity. Mater Today Chem, 32, 2024, 101330 https://doi.org/10.1016/j.mtchem.2024.101330
Rajendran S, Priyadarshini E, Rajeshkumar S. Microwave-assisted synthesis of ZnO nanoparticles using Annona squamosa peel extract and their biomedical applications. J Mol Struct, 1308, 2024, 136597 https://doi.org/10.1016/j.molstruc.2024.136597
Khan SA, Nadeem M, Hussain T, Ahmad A. Ultrasonication-assisted green synthesis of zinc oxide nanoparticles from Moringa oleifera leaf extract: Characterisation and antimicrobial activity. Ultrason Sonochem, 97, 2024, 106584 https://doi.org/10.1016/j.ultsonch.2024.106584
Ali M, Alobaid A, Alharbi NS, Kadaikunnan S, Khan KA, Alzohairy MA. Ultrasound-assisted synthesis of ZnO nanoparticles using Ocimum basilicum extract: Evaluation of cytotoxicity and antioxidant activity. Arab J Chem, 17(8), 2024, 105761 https://doi.org/10.1016/j.arabjc.2024.105761
Das S, Mitra S, Mandal A, Banerjee A. Comparative evaluation of co-precipitation and green synthesis routes for ZnO nanoparticles: Structural, optical, and antimicrobial studies. Mater Chem Phys, 317, 2025, 129064 https://doi.org/10.1016/j.matchemphys.2025.129064
Patel N, Shah P, Joshi M, Khatri P. Structural and biopharmaceutical evaluation of zinc oxide nanoparticles synthesised via co-precipitation method: Effect of calcination on particle morphology. J Nanostruct Chem, 15(2), 2025, 455–468 https://doi.org/10.1007/s40097-025-00811-3
Singh P, Kim YJ, Zhang D, Yang DC. Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol, 34(7), 2016, 588–599 https://doi.org/10.1016/j.tibtech.2016.02.006
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