Development and in vitro evaluation of liquid crystal-based polyherbal hair gels: physicochemical characterization, hair performance, and antioxidant assessment
DOI:
https://doi.org/10.69857/joapr.v13i4.1225Keywords:
Liquid crystal gel, polyherbal formulation, hair care, anti-frizz, antioxidant activity, cosmeceuticalsAbstract
Background: A liquid crystal (LC) based polyherbal hair gel was developed to enhance physicochemical stability and functional performance in topical hair care. The objective was to integrate herbal oils (flaxseed, coconut, and almond) and aqueous extracts (green tea, keratin hydrolysate, and pea peptide), known for their moisturizing, antioxidant, follicle-protective, and anti-frizz effects, into a stable gel matrix for scalp care and conditioning. Methodology: Ten formulations (F1–F10) incorporating flaxseed, coconut, and almond oils with green tea, marula extract (Sclerocarya birrea), keratin hydrolysate, and pea extract were prepared via coacervation, vortex mixing, and high-pressure homogenization. The gels were evaluated for their organoleptic properties, pH, spreadability, particle size (as determined by dynamic light scattering, DLS), polydispersity index (PDI), and zeta potential. Polarized Light Microscopy and FTIR characterized structural features. Functional performance was evaluated by in vitro studies on hair diameter and weight changes, as well as anti-frizz, anti-static, and antioxidant (DPPH) activities. Results and Discussion: Formulation F6 showed optimal nanometric characteristics (186.47 ± 1.90 nm, PDI 0.351 ± 0.01, zeta potential −35.9 mV), indicating stable colloidal dispersion. FTIR and microscopy confirmed molecular compatibility and mesophase birefringence. In vitro assessments revealed marked improvement in hair thickness for F6 and F9, with superior anti-frizz and anti-static performance for F4 and F9. Antioxidant activity was moderate compared to Trolox. F4 and F6 maintained stability over 28 days at different temperatures. Conclusion: F4 and F6 demonstrated superior in vitro performance and stability, suggesting promise as cosmeceutical hair care candidates. In vivo and clinical studies are required to confirm efficacy and long-term safety.
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References
Janhavi Yogiraj Bure, Rohan Dilip Morpake, Ketan Sunil Uike, Shilpa V. Jaiswal, Dr. M. D. Kitukale. Bioactive Compounds in Herbal Shampoos: A Comprehensive Review of their Efficacy in Promoting Hair Growth and Controlling Dandruff. IJARSCT, 533–41 (2025) https://doi.org/10.48175/IJARSCT-23063.
Ahmed A, Alali AM, Abdullah E, Alharbi MN, Alayoubi HM. Herbal Remedies for Hair Loss: A Review of Efficacy and Safety. Skin Appendage Disord, 1–15 (2025) https://doi.org/10.1159/000542876.
Sindhuri GV, Navya B, Ujwala K, Reshma S, Tejaswini S, Vaishnavi S. Review On Herbs: International Guidelines, Future Insights and Patenting of Herbals. IJFMR - International Journal for Multidisciplinary Research, 6, (2024) https://doi.org/10.36948/ijfmr.2024.v06i06.33532.
David SR, Rajabalaya R. Development and in vitro Evaluation of Nanoemulsion-based Polyherbal Hair Serum Formulations for Leave-in Hair Treatments. COSCI, 03, e26667797319847 (2024) https://doi.org/10.2174/0126667797319847240910105239.
Bhat YJ, Saqib N-U-, Latif I, Hassan I. Female Pattern Hair Loss—An Update. Indian Dermatology Online Journal, 11, 493 (2020) https://doi.org/10.4103/idoj.IDOJ_334_19.
Bazmi S, Sepehrinia M, Pourmontaseri H, Bazyar H, Vahid F, Farjam M, Dehghan A, Hébert JR, Homayounfar R, Shakouri N. Androgenic alopecia is associated with higher dietary inflammatory index and lower antioxidant index scores. Front. Nutr., 11, 1433962 (2024) https://doi.org/10.3389/fnut.2024.1433962.
Shoko T, Maharaj VJ, Naidoo D, Tselanyane M, Nthambeleni R, Khorombi E, Apostolides Z. Anti-aging potential of extracts from Sclerocarya birrea (A. Rich.) Hochst and its chemical profiling by UPLC-Q-TOF-MS. BMC Complement Altern Med, 18, 54 (2018) https://doi.org/10.1186/s12906-018-2112-1.
Bello OO, Akande RO, Bello TK, Fashola MO, Oni MO, Osho A. Phytochemical and Antifungal Evaluations of Virgin Cocos nucifera (Coconut) Oil. OBM Integr Complement Med, 10, 1–15 (2025) https://doi.org/10.21926/obm.icm.2501006.
Lal Baghel K, Kumar R, Dewangan P, Kumar Nema R, Wamankar S. A Comprehensive Review of “Herbal Hair Oil: A Natural Remedy for Hair Loss and Scalp Health”. IJAMRS, 4, 1034–7 (2024) https://doi.org/10.62225/2583049X.2024.4.6.3547.
Fan J, Wu L, Wang J, Bian X, Chen C, Chang K. Performance and Mechanism of Hydrolyzed Keratin for Hair Photoaging Prevention. Molecules, 30, 1182 (2025) https://doi.org/10.3390/molecules30051182.
Grothe T, Wandrey F, Schuerch C. Short communication: Clinical evaluation of pea sprout extract in the treatment of hair loss. Phytotherapy Research, 34, 428–31 (2020) https://doi.org/10.1002/ptr.6528.
Teeranachaideekul V, Soontaranon S, Sukhasem S, Chantasart D, Wongrakpanich A. Influence of the emulsifier on nanostructure and clinical application of liquid crystalline emulsions. Sci Rep, 13, 4185 (2023) https://doi.org/10.1038/s41598-023-31329-w.
Nath AG, Dubey P, Kumar A, Vaiphei KK, Rosenholm JM, Bansal KK, Gulbake A. Recent Advances in the Use of Cubosomes as Drug Carriers with Special Emphasis on Topical Applications. Journal of Lipids, 2024, 2683466 (2024) https://doi.org/10.1155/2024/2683466.
Han X, Yan Q, Gao N, Kang Y, Li N, Zou A. Biocompatible Lyotropic Nanocarriers for Improved Delivery of Ascorbyl Tetraisopalmitate in Skincare. Langmuir, 41, 3278–90 (2025) https://doi.org/10.1021/acs.langmuir.4c04187.
Rajabalaya R, Musa MN, Kifli N, David SR. Oral and transdermal drug delivery systems: role of lipid-based lyotropic liquid crystals. DDDT, 11, 393–406 (2017) https://doi.org/10.2147/DDDT.S103505.
Adeline D, Michel G, Ecaterina G. Cosmetic emulsions containing innovative complex coacervates: A cross-sectional study. International Journal of Cosmetic Science, 47, 383–97 (2025) https://doi.org/10.1111/ics.13035.
Guerrero-Vasquez FJ, Ortega F, Rubio RG, Guzmán E. Bio-Based Interpolyelectrolyte Complexes for the Stabilization of Pickering-like Emulsions. Colloids and Interfaces, 9, 9 (2025) https://doi.org/10.3390/colloids9010009.
Aliudin NA, David SR, Rajabalaya R. Formulation and in Vitro Efficacy Evaluation of Polyherbal Hair Tonics for Enhancing Hair Health on Various Hair Types. Biomedical and Pharmacology Journal, 17, 2643–60 (2024) https://doi.org/10.13005/bpj/3055.
Rajabalaya R, Leen G, Chellian J, Chakravarthi S, David S. Tolterodine Tartrate Proniosomal Gel Transdermal Delivery for Overactive Bladder. Pharmaceutics, 8, 27 (2016) https://doi.org/10.3390/pharmaceutics8030027.
Musa MN, David SR, Zulkipli IN, Mahadi AH, Chakravarthi S, Rajabalaya R. Development and evaluation of exemestane-loaded lyotropic liquid crystalline gel formulations. Bioimpacts, 7, 227–39 (2017) https://doi.org/10.15171/bi.2017.27.
Yusof N, David SR, Mumin NH, Ahmad L, Rajabalaya R. Formulation and development of Commiphora myrrha based polyherbal nanoemulsion mouthwash and assessment of its anti-oxidant and cytotoxicity activity. Journal of Applied Pharmaceutical Research, 12, 87–101 (2024) https://doi.org/10.69857/joapr.v12i5.679.
Mahdi WA, Imam SS, Alotaibi A, Alhallaf S, Alzhrani RF, Alshehri S. Formulation and Evaluation of a Silymarin Inclusion Complex-Based Gel for Skin Cancer. ACS Omega, 10, 3006–17 (2025) https://doi.org/10.1021/acsomega.4c09614.
Darshini D, Likhita B, Mohitha YB, Kumari VA, Hema M, Ahasanuzzaman D. Formulation and Evaluation of Liposomal Cream Containing Tolnaftate. Journal of Drug Delivery and Therapeutics, 15, 13–8 (2025) https://doi.org/10.22270/jddt.v15i1.6944.
Rajabalaya R, David SR, Chellian J, Xin Yun G, Chakravarthi S. Transdermal delivery of oxybutynin chloride proniosomal gels for the treatment of overactive bladder. Drug Delivery, 23, 1578–87 (2016) https://doi.org/10.3109/10717544.2015.1116027.
David SR, Matzidi FNNH, Shanmugam R, Thangavelu L, Balaraman AK, Rajabalaya R. Evaluation of the Chemo-Preventive Effects of Camellia sinensis Silver Nanoparticles and Synergistic Effects with 5-fluorouracil in Colorectal Cancer Induced Rats. Current Trends in Biotechnology and Pharmacy, 16, 114–29 (2022) https://doi.org/10.5530/ctbp.2022.2.12.
Abla KK, Alamoudi MK, Soliman GA, Abdel-Kader MS, Aldawsari MF, Mehanna MM. Alopecia Management Potential of Rosemary-Based Nanoemulgel Loaded with Metformin: Approach Combining Active Essential Oil and Repurposed Drug. IJN, 20, 605–24 (2025) https://doi.org/10.2147/IJN.S500487.
Gauri Paratkar, Surekha Bhosale, Asmita Zodage. Formulation and Evaluation of Polyherbal Anti-Aging Gel from Clitoria Ternatea and Jasmine Extract. IJARSCT, 574–8 (2025) https://doi.org/10.48175/IJARSCT-23067.
David SRN, Mohammad MS, Chee LY, Rajabalaya R. Is Sunflower Cooking Oil Beneficial for Colorectal Cancer? In Vivo Studies on Azoxymethane-induced Colon Cancer in Rats. CNF, 18, 329–36 (2022) https://doi.org/10.2174/1573401317666211208144625.
Bejenaru LE, Segneanu A-E, Bejenaru C, Bradu IA, Vlase T, Herea D-D, Văruţ MC, Bălăşoiu RM, Biţă A, Radu A, Mogoşanu GD, Ciocîlteu MV. Thermoresponsive Gels with Rosemary Essential Oil: A Novel Topical Carrier for Antimicrobial Therapy and Drug Delivery Applications. Gels, 11, 61 (2025) https://doi.org/10.3390/gels11010061.
Kartik K. Wankhede, Shivani Suryawanshi, Dr. M. D. Kitukale. Development and Evaluation of Herbal Pain Relief Gel. IJARSCT, 370–3 (2025) https://doi.org/10.48175/IJARSCT-23043.
Sánchez-Tito M, Tay LY, Zea-Gamboa F, Cartagena-Cutipa R, Flores-Gómez A, Spigno-Paco B, Salinas BRM, Calcina JEZ, Díaz IEC. Development and Evaluation of the Antibacterial Properties of an Experimental Herbal Gel Against Cariogenic Bacteria. Clinical and Experimental Dental Research, 11, e70076 (2025) https://doi.org/10.1002/cre2.70076.
Lukić M, Pantelić I, Savić SD. Towards Optimal pH of the Skin and Topical Formulations: From the Current State of the Art to Tailored Products. Cosmetics, 8, 69 (2021) https://doi.org/10.3390/cosmetics8030069.
Bodalska A, Kowalczyk A, Fecka I. Stability of Rosmarinic Acid and Flavonoid Glycosides in Liquid Forms of Herbal Medicinal Products—A Preliminary Study. Pharmaceuticals, 14, 1139 (2021) https://doi.org/10.3390/ph14111139.
Balkrishna A, Ngpoore NK, Jonwal H, Lochab S, Varshney A. Anti-furfurative comparison of Kesh Kanti-Herbal Shampoos and synthetic shampoos against Malassezia furfur for dandruff management. AMB Express, 15, 8 (2025) https://doi.org/10.1186/s13568-024-01818-w.
Jung S-W, Park GH, Kim E, Yoo KM, Kim HW, Lee JS, Chang MY, Shin K-O, Park K, Choi EH. Rosmarinic Acid, as an NHE1 Activator, Decreases Skin Surface pH and Improves the Skin Barrier Function. International Journal of Molecular Sciences, 23, 3910 (2022) https://doi.org/10.3390/ijms23073910.
Gaikwad MT, Marathe RP. Cilnidipine-loaded transdermal nanoemulsion-based gel: synthesis, optimisation, characterisation, and pharmacokinetic evaluation. International Journal of Applied Pharmaceutics, 255–74 (2025) https://doi.org/10.22159/ijap.2025v17i1.52689.
Bodnár K, Papp B, Sinka D, Fehér P, Ujhelyi Z, Lekli I, Kajtár R, Nacsa F, Bácskay I, Józsa L. Development of Salvia officinalis–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration Enhancement. Pharmaceutics, 17, 140 (2025) https://doi.org/10.3390/pharmaceutics17020140.
Liu M, Sharma M, Lu G, Zhang Z, Song W, Wen J. Innovative Solid Lipid Nanoparticle-Enriched Hydrogels for Enhanced Topical Delivery of L-Glutathione: A Novel Approach to Anti-Ageing. Pharmaceutics, 17, 4 (2025) https://doi.org/10.3390/pharmaceutics17010004.
Zhang X, Wu C-C, Jiang H, Zhao J-F, Pan Z-J, Zheng Y. The Role of Thickening Agent Proportions in Optimizing Nanoemulsion Gel for Dermatophytosis Treatment. IJN, 20, 807–26 (2025) https://doi.org/10.2147/IJN.S479514.
Wongsa P, Phatikulrungsun P, Prathumthong S. FT-IR characteristics, phenolic profiles and inhibitory potential against digestive enzymes of 25 herbal infusions. Sci Rep, 12, 6631 (2022) https://doi.org/10.1038/s41598-022-10669-z.
Singh A, Srivastav N. Phytomolecules to Improve Skin Health: A polyherbal Emulgel Formulation. Journal of Advanced Zoology, 44, 1008–18 (2023) https://doi.org/10.17762/jaz.v44iS-5.1085.
Vandanjon L, Burlot A-S, Zamanileha EF, Douzenel P, Ravelonandro PH, Bourgougnon N, Bedoux G. The Use of FTIR Spectroscopy as a Tool for the Seasonal Variation Analysis and for the Quality Control of Polysaccharides from Seaweeds. Marine Drugs, 21, 482 (2023) https://doi.org/10.3390/md21090482.
Sun M, Deng Y, Cao X, Xiao L, Ding Q, Luo F, Huang P, Gao Y, Liu M, Zhao H. Effects of Natural Polyphenols on Skin and Hair Health: A Review. Molecules, 27, 7832 (2022) https://doi.org/10.3390/molecules27227832.
Song S-H, Lim JH, Son SK, Choi J, Kang N-G, Lee S-M. Prevention of lipid loss from hair by surface and internal modification. Sci Rep, 9, 9834 (2019) https://doi.org/10.1038/s41598-019-46370-x.
Wang W, Wang H, Luo Y, Li Z, Li J. Discovery of petroleum ether extract of eclipta targeting p53/Fas pathway for the treatment of chemotherapy-induced alopecia: Network pharmacology and experimental validation. Journal of Ethnopharmacology, 333, 118405 (2024) https://doi.org/10.1016/j.jep.2024.118405.
Csuka DA, Csuka EA, Juhász MLW, Sharma AN, Mesinkovska NA. A systematic review on the lipid composition of human hair. International Journal of Dermatology, 62, 404–15 (2023) https://doi.org/10.1111/ijd.16109.
Coderch L, Alonso C, García MT, Pérez L, Martí M. Hair Lipid Structure: Effect of Surfactants. Cosmetics, 10, 107 (2023) https://doi.org/10.3390/cosmetics10040107.
Hartson M, Coyle C, Amin S. Methylcellulose-Chitosan Smart Gels for Hairstyling. Cosmetics, 9, 69 (2022) https://doi.org/10.3390/cosmetics9040069.
Choi HY, Lee YJ, Kim CM, Lee Y-M. Revolutionizing Cosmetic Ingredients: Harnessing the Power of Antioxidants, Probiotics, Plant Extracts, and Peptides in Personal and Skin Care Products. Cosmetics, 11, 157 (2024) https://doi.org/10.3390/cosmetics11050157.
Khan N, Jadhav P, Jagadale S, Kachare K, Kamble S, Gandhe S, Yewale R, Kale DrM, Kshirsagar S. Formulation and evaluation of polyherbal hair gel formulation. J Pharmacogn Phytochem, 11, 32–6 (2022) https://doi.org/10.22271/phyto.2022.v11.i1a.14318.
Mallamma T, Kumar BP, Suchindar A, Pragathi Y, Meghana C, Hemalatha KJ. Formulation and Performance of Polyherbal Antidandruff Hair Gel: A Multifaceted Solution. Asian Journal of Pharmaceutical Research and Health Care, 16, 67 (2024) https://doi.org/10.4103/ajprhc.ajprhc_119_23.
Zhang L, Shen B, Zheng C, Huang Y, Liang Y, Fei P, Chen J, Lai W. Chitosan/oxidized sodium alginate/Ca2+ hydrogels: Synthesis, characterization and adsorption properties. Food Hydrocolloids, 156, 110368 (2024) https://doi.org/10.1016/j.foodhyd.2024.110368.
Hartanti D, Hamad A. Antioxidant properties and interaction effects of a novel polyherbal formulation. Current Trends in Biotechnology and Pharmacy, 17, 28–33 (2023) https://doi.org/10.5530/ctbp.2023.4s.87.
Putra WH, Subekti S, Amin M. In Vitro Antioxidant Activity Dpph Assay of Protein Hydrolysate Mackerel (Scomber Scombrus) Viscera. Grouper, 15, 244–53 (2024) https://doi.org/10.30736/grouper.v15i2.266.
R David S, Ahad NI, Balaraman AK, Rajabalaya R. Development and Evaluation of Sulfate-Free Charcoal Toothpaste from Coconut Shell and Rice husk. Curr. Trends Biotechnol. Pharm, 18, 1850–65 (2024) https://doi.org/10.5530/ctbp.2024.3.32.
Gautam A, Upadhyay S. Formulation and Standardization of Polyherbal Face Wash Gel for Acne Management. Research Journal of Pharmacy and Technology, 15, 3931–5 (2022) https://doi.org/10.52711/0974-360X.2022.00658.
Rani S, Manju SL. HPLC Quantification and Stability Study of Antilipidemic Polyherbal Formulation. Journal of Natural Remedies, 380–92 (2022) https://doi.org/10.18311/jnr/2022/29013.
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