Formulation and characterisation of polyherbal syrup against aldose reductase enzyme
DOI:
https://doi.org/10.69857/joapr.v14i2.1189Keywords:
Diabetes Mellitus, Advanced Glycation End Products, Antiglycation Activity, Antioxidant Properties, Herbal FormulationsAbstract
Background: Traditional medicinal plants have been suggested to help regulate blood glucose levels and inhibit glycation. This investigation aims to formulate and evaluate the potential of herbal formulations containing plant extracts that may inhibit Advanced glycation end product (AGE) formation and provide therapeutic advantages in the management of diabetes. Methodology: The herbal syrup containing polyherbal extracts of onion, garlic, and cinnamon was developed at varying concentrations with a viscosity modifier, preservative, flavoring agent, and other excipients. The developed syrup formulations were physicochemically characterised, including antioxidant potential, an ALR (aldose reductase) inhibition assay, and a stability study. Results and Discussion: The formulated herbal syrup exhibited a dark brown colour with a slightly Pungent taste. The pH of the syrup was measured at 6.48, indicating a mildly acidic to neutral nature. The viscosity of the prepared herbal syrup formulations (F1–F4) ranged from 3.71 to 3.80 cP. All formulations exhibited concentration-dependent antioxidant activity. F3 demonstrated the highest antioxidant activity and aldose reductase inhibition with 59.58% and 66.44% inhibition at 1000 μg/mL, respectively. Overall, the findings suggest that the herbal syrup formulation exhibited excellent physical and chemical stability over 3 months. Conclusion: F3 formulation may be a valuable candidate for the prevention and management of oxidative stress-related disorders, including diabetic complications, owing to its notable aldose reductase enzyme activity and potent antioxidant potential.
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References
Aebi M. N-linked protein glycosylation in the ER. Biochim Biophys Acta Mol Cell Res, 1833, 2430–7 (2013) https://doi.org/10.1016/j.bbamcr.2013.04.001
Ahmed N. Advanced glycation endproducts—role in pathology of diabetic complications. Diabetes Res Clin Pract, 67, 3–21 (2005) https://doi.org/10.1016/j.diabres.2004.09.004
Asgharpour Dil F, Ranjkesh Z, Goodarzi MT. A systematic review of antiglycation medicinal plants. Diabetes Metab Syndr, 13, 1225–9 (2019) https://doi.org/10.1016/j.dsx.2019.01.053
Sell DR, Lapolla A, Odetti P, Fogarty J, Monnier VM. Pentosidine formation in skin correlates with severity of complications in individuals with long-standing IDDM. Diabetes, 41, 1286–92 (1992) https://doi.org/10.2337/diab.41.10.1286
Dudhe B, Kamdi N, Giradkar A, Astankar P, Mankar N, Ghotkar U. Assessment of knowledge, attitude, and practices on antibiotic use and its resistance among medical students in tertiary care teaching hospitals of Maharashtra. J Appl Pharm Res, 11(5), 26–33 (2023) https://doi.org/10.18231/j.joapr.2023.11.5.26.33
Tsukahara H, Sekine K, Uchiyama M, Kawakami H, Hata I, Todoroki Y, Hiraoka M, Kaji M, Yorifuji T, Momoi T, Yoshihara K, Beppu M, Mayumi M. Formation of advanced glycosylation end products and oxidative stress in young patients with type 1 diabetes. Pediatr Res, 54, 419–24 (2003) https://doi.org/10.1203/01.PDR.0000076662.72100.74
PRD P. In vitro antiglycation activity of some medicinal plants used in diabetes mellitus. Med Aromat Plants (Los Angel), 2, 143 (2013) https://doi.org/10.4172/2167-0412.1000143
Ahmad MS, Ahmed N. Antiglycation properties of aged garlic extract: possible role in prevention of diabetic complications. J Nutr, 136, 796S–799S (2006) https://doi.org/10.1093/jn/136.3.796S
Tomlinson DR, Stevens EJ, Diemel LT. Aldose reductase inhibitors and their potential for the treatment of diabetic complications. Trends Pharmacol Sci, 15, 293–7 (1994) https://doi.org/10.1016/0165-6147(94)90010-8
Shiels A, Hejtmancik JF. Biology of inherited cataracts and opportunities for treatment. Annu Rev Vis Sci, 5, 123–149 (2019) https://doi.org/10.1146/annurev-vision-091517-034346
Lee AYW, Chung SSM. Contributions of polyol pathway to oxidative stress in diabetic cataract. FASEB J, 13, 23–30 (1999) https://doi.org/10.1096/fasebj.13.1.23
El-Kabbani O, Ruiz F, Darmanin C, Chung RP-T. Aldose reductase structures: implications for mechanism and inhibition. Cell Mol Life Sci, 61, 750–62 (2004) https://doi.org/10.1007/s00018-003-3403-2
Hamada Y, Araki N, Koh N, Nakamura J, Horiuchi S, Hotta N. Rapid formation of advanced glycation end products by intermediate metabolites of glycolytic pathway and polyol pathway. Biochem Biophys Res Commun, 228, 539–43 (1996) https://doi.org/10.1006/bbrc.1996.1695
Lokeshvar R, Ramaiyan V, Nithin V, Pavani S, Vinod Kumar T. Nanotechnology-driven therapeutics for liver cancer: clinical applications and pharmaceutical insights. Asian J Pharm Clin Res, 18(2), 8–26 (2025) https://doi.org/10.22159/ajpcr.2025v18i2.53429
Hwang SH, Kim H-Y, Guillen Quispe YN, Wang Z, Zuo G, Lim SS. Aldose reductase, protein glycation inhibitory and antioxidant of Peruvian medicinal plants: the case of Tanacetum parthenium L. and its constituents. Molecules, 24, 2010 (2019) https://doi.org/10.3390/molecules24102010
Chang K-C, Shieh B, Petrash JM. Role of aldose reductase in diabetes-induced retinal microglia activation. Chem Biol Interact, 302, 46–52 (2019) https://doi.org/10.1016/j.cbi.2019.01.020
Akileshwari C, Raghu G, Muthenna P, Mueller NH, Suryanarayana P, Petrash JM, Reddy GB. Bioflavonoid ellagic acid inhibits aldose reductase: implications for prevention of diabetic complications. J Funct Foods, 6, 374–83 (2014) https://doi.org/10.1016/j.jff.2013.11.004
Veeresham C, Rama Rao A, Asres K. Aldose reductase inhibitors of plant origin. Phytother Res, 28, 317–33 (2014) https://doi.org/10.1002/ptr.5000
Kato A, Yasuko H, Goto H, Hollinshead J, Nash RJ, Adachi I. Inhibitory effect of rhetsinine isolated from Evodia rutaecarpa on aldose reductase activity. Phytomedicine, 16, 258–61 (2009) https://doi.org/10.1016/j.phymed.2007.04.008
Kubo M, Matsuda H, Tokuoka K, Kobayashi Y, Ma S, Tanaka T. Studies of anti-cataract drugs from natural sources. I. Effects of a methanolic extract and the alkaloidal components from Corydalis tuber on in vitro aldose reductase activity. Biol Pharm Bull, 17, 458–9 (1994) https://doi.org/10.1248/bpb.17.458
Thakur S, Gupta SK, Ali V, Singh P, Verma M. Aldose reductase: a cause and a potential target for the treatment of diabetic complications. Arch Pharm Res, 44(7), 655–67 (2021) https://doi.org/10.1007/s12272-021-01343-5
Hsu C, Yang H, Ho J, Yin M, Hsu J. Houttuynia cordata aqueous extract attenuated glycative and oxidative stress in heart and kidney of diabetic mice. Eur J Nutr, 55, 845–54 (2016) https://doi.org/10.1007/s00394-015-0994-y
Singh M, Kapoor A, Bhatnagar A. Physiological and pathological roles of aldose reductase. Metabolites, 11(10), 655 (2021) https://doi.org/10.3390/metabo11100655
Vikhe S, Sukhadhane P, Vikhe R, Bornare SL, Dhavane SS. Antidiabetic effects of Semecarpus anacardium leaf extracts in streptozotocin-induced diabetes in rats. J Appl Pharm Res, 12(6), 144–58 (2024) https://doi.org/10.69857/joapr.v12i6.736
Balunas MJ, Kinghorn AD. Drug discovery from medicinal plants. Life Sci, 78, 431–41 (2005) https://doi.org/10.1016/j.lfs.2005.09.012
Verma AK, Dewangan K, Daunday L, Naurange K, Verma K, Bhiaram M. Spirulina as functional food: insights into cultivation, production, and health benefits. J Appl Pharm Res, 12(5), 28–50 (2024) https://doi.org/10.69857/joapr.v12i5.788
Antony P, Vijayan R. Identification of novel aldose reductase inhibitors from spices: a molecular docking and simulation study. PLoS One, 10, e0138186 (2015) https://doi.org/10.1371/journal.pone.0138186
Eze IL, Onyegbule FA, Ezugwu CO, Chibuzor JV, Aziakpono OM. Phytochemical and antioxidant evaluations of Chromolaena odorata and Huntaria umbellata. J Pharm Res Int, 36, 213–24 (2024) https://doi.org/10.9734/jpri/2024/v36i127641
Shaikh P, Lokare M, Bhoge M, Rajmane N, Avadhut N, Thavare N. Formulation and evaluation of antidiabetic polyherbal syrup. J Pharmacogn Phytochem, 13, 10–7 (2024) https://doi.org/10.22271/phyto.2024.v13.i2a.14865
Kondhare D, Lade H. Phytochemical profile, aldose reductase inhibitory, and antioxidant activities of Indian traditional medicinal Coccinia grandis (L.) fruit extract. 3 Biotech, 7, 378 (2017) https://doi.org/10.1007/s13205-017-1013-1
Haraguchi H, Ohmi I, Sakai S, Fukuda A, Toihara Y, Fujimoto T, Okamura N, Yagi A. Effect of Polygonum hydropiper sulfated flavonoids on lens aldose reductase and related enzymes. J Nat Prod, 59, 443–5 (1996) https://doi.org/10.1021/np9601622
Srivastava S, Upadhye VJ, Prasad ME, Singh P. Applications of bioactive compounds of traditional Chinese medicine in breast cancer management. J Appl Pharm Res, 13(4), 1–15 (2025) https://doi.org/10.69857/joapr.v13i4.935
Lad V, Ahmad S. Development, standardization and evaluation of antimicrobial syrup. Int J Drug Deliv Technol, 14, 389–95 (2024) https://doi.org/10.25258/ijddt.14.1.57
Grewal AS, Thapa K, Kanojia N, Sharma N, Singh S. Natural compounds as source of aldose reductase (AR) inhibitors for the treatment of diabetic complications: a mini review. Curr Drug Metab, 21(14), 1091–1116 (2020) https://doi.org/10.2174/1389200221666201016124125
Maccari R, Ottanà R. Targeting aldose reductase for the treatment of diabetes complications and inflammatory diseases: new insights and future directions. J Med Chem, 58(5), 2047–67 (2015) https://doi.org/10.1021/jm500907a
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