Mycotic loads’ determination of non-sterile pharmaceuticals in Lagos state and 16S RDNA identification of the fungal isolates

Elizabeth Olawumi  Oyebanji; Adedotun Adeyinka Adekunle; Herbert Alexander B. Coker; Gboyega Ebenezer Adebami

doi:10.18231/2348-0335.2018.0007

Authors

Elizabeth Olawumi Oyebanji Department of Botany, University of Lagos, Nigeria
Adedotun Adeyinka Adekunle Department of Botany, University of Lagos, Nigeria
Herbert Alexander B. Coker Department of Pharmaceutical Chemistry, Faculty of Pharmacy, College of Medicine Campus, Idiaraba, University of Lagos
Gboyega Ebenezer Department of Biological Sciences, Mountain Top University, Ibafo, Ogun State

DOI:

https://doi.org/10.18231/2348-0335.2018.0007

Keywords:

Oral drug, Contamination, Fungal load, Fungal identification, Colony forming unit, Aspergillus

Abstract

Pharmaceuticals are medicinal products used in the prevention, treatment, and diagnosis of diseases. As such, the presence of microorganisms’ especially fungal toxins can reduce or eliminate the product's therapeutic activity and constitute a potential danger to patient health. This study attempts to determine the fungal loads of liquid preparation used as medication in Lagos State and its environs and identify the fungal isolates. 252 different types of oral liquid drugs (200syrups and 52 suspensions) which included paracetamol syrup, cough syrup and antibiotics suspension manufactured in five different pharmaceutical industries in Nigeria were methodically sampled and analyzed for fungi contamination using standardized method. The isolated fungi were identified using morphological characterization as well as 16SrDNA sequencing. 13% of the sampled syrups were found to be contaminated with fungi where thecolony forming units for paracetamol syrup, cough syrup and antibiotics suspension ranges from 2.0x10¹- 9.6×10⁴; 1.0x10²- 7.5x10⁴ and 1.0x10¹- 8.8×10⁴cfu/ml respectively while87% yielded no growth.Thirteen (13) morphologically different species of fungi were identified which included Aspergillus niger strains, A. tamari strains, A. japonicas, A. flavus, A. awamari, A. ellipticus, A. tubingensis, Meyerozyma aaribbica, Candida carpophila and Eurotiomycetes spp, The presence of microorganisms in oral liquid samples might explain the treatment complicacy of the diseased children. Therefore, microbiological quality of such drugs is thus suggested.

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